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About Us

Our laboratory highlights on the realization of cutting-edge findings of basic researches from collaborating world-leading facilities, or investigating novel therapeutic modalities or devices, for severe cardiovascular diseases resistant to conventional therapies. In the field of translational research, there is a phrase “The valley of death”, which indicates the situation that patients could not survive without having excellent therapies brought from basic researches because of the lack of personnel who transport the knowledge to suffering patients. We pursue to be the personnel who bridge the gap, making the best use of our surgical skill and point of view. We work for the patients.

Main Projects

1. ES/iPS cell-based regenerative medicine

We have collaborated with Center for iPS Cell Research and Application (CiRA), Kyoto University for the researches of ES/iPS cell-based regenerative medicine. Combining ES/iPS cell differentiation system to cardiovascular cell populations (Prof. Jun K Yamashita, CiRA) and cell sheet technology (Institute of Advanced Biomedical and Science, Tokyo Women’s Medical University), we have shown excellent therapeutic potential of ES/iPS cell-derived cardiac cell sheets1.
We are now investigating for the molecular and cellular mechanisms of cell-based cardiac regenerative therapy using this ES/iPS cell-derived cardiac sheet system, and expanding this technology to human iPS cells for future clinical application.

Mouse ES cell-derived cardiomyocytes (left, center)11, endothelial and vascular mural cells (right)1.

Mouse ES cell-derived cardiac cell sheets (left) and the transplantation to rat sub-acute myocardial infarction model (center, right)1.

Neovascularization induced from cell sheet transplantation. vWF-positive (green) endothelial cells prominently accumulated around the grafted area (left) leading to increased capillary density (right)1.

2. “Bridge-to-recovery” from left ventricular assist devices (LVAD) for failing hearts

We have investigated therapeutic methods for severe heart failure needed mechanical support such as left ventricular assist devices (LVAD). This theme is especially important for Japanese society because of limited opportunity for heart transplantation. We established novel rat heterotopic heart transplantation model which unload the volume of failing heart, simulating LVAD situation1,3,6,8. We are investigating effective multidisciplinary approach for this refractory pathophysiology, including sustained release of anti-fibrotic or anti-apoptotic cytokines (e.g. hepatocyte growth factor) using gelatin hydrogel.

3. Angiogenic approach for severe limb ischemia using sustained release of basic fibro
 blast growth factor (bFGF) with gelatin hydrogel

We have investigated a novel angiogenic approach for severe peripheral arterial diseases (PAD) utilizing sustained release of basic fibroblast growth factor (bFGF),a potent angiogenic cytokine, incorporated to gelatin hydrogel (collaboration with Prof. Yasuhiko Tabata, Institute for Frontier Medical Sciences, Kyoto University)26,27,28,33,34.

Preclinical study of bFGF therapy for rabbit hind limb ischemia. Angiography of control (left) and bFGF-treated rabbit (right)27.

A representative result of bFGF therapy for severe PAD (phase I-IIa clinical study). Pre-treatment (left) and 12 weeks after treatment (right)26.

4. Development of new devices for endovascular surgery

We have developed new devices for safer endovascular surgery using large animal model (e.g. porcine, canine) such as detachable arterial filter to prevent thromboembolism during endovascular surgery2, or side-branched stent graft for the perfusion of the artery of Adamkiewicz to prevent paraplegia after thoracic aortic stenting10. The experimental setting is almost the same as human surgery, and we can fully apply our surgical technique in this experimental field.

Experimental procedure using canine model.

The attachment of detachable arterial filter into internal carotid artery (scheme) (left) and the angiography after attachment (right)2.

The Inoue stent graft with small caliber branch for the perfusion of the artery of Adamkiewicz10.

Other Projects

  • 1.The application of novel biodegradable glue derived from food additives for prevention of retrosternal adhesion, the career for antiarrhythmic agents5, or antibiotics6.
  • 2. The cardioprotective effects of statin or polyphenol3,35 using rat Langendorff apparatus.
  • 3. Attenuation of LV remodeling after LV repair using rat OMI model8,17,18,36,41,42,43.
  • 4. Organ protection during cardiopulmonary bypass (CPB) using rat CPB model.
  • 5. Attenuation of reperfusion injury after recanalization of acute lower limb thromboembolism9.
  • 6. The application of micro-RNAs for the treatment of cardiovascular diseases.
  • 7. Angiogenic effect of platelet-rich plasma (PRP)4,13.
  • 8. New operative technique for LV plasty40 or repair of mitral valve22,23,24,29,39 using canine CPB model.
  • 9. RV function after surgical repair of congenital heart diseases using canine model.

Collaborators

Kyoto University
Center for iPS Cell Research and Application (CiRA)
Institute for Frontier Medical Sciences
Department of Cardiovascular medicine
Department of Nephrology
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University
National Cerebral and Cardiocvascular Center
Kyoto Institute of Technology

Welcome to foreign students or researchers

We welcome foreign visitors for our research laboratory.
Please contact us.

Selected Publication List

Contact Information

Hidetoshi Masumoto, M.D, Ph.D.
Assistant Professor
Department of Cardiovascular Surgery,
Kyoto University Hospital

54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, JAPAN.
Tel: +81-75-751-3784 Fax: +81-75-751-4960
masumoto@kuhp.kyoto-u.ac.jp